SH3PX1 Polyclonal Antibody, PE-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12407R-PE-Cy7-100uL |
| Product Name | SH3PX1 Polyclonal Antibody, PE-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | PE-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SNX9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51429 |
| Swiss Prot | Q9Y5X1 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | SDP1; WISP; SH3PX1; SH3PXD3A; Sorting nexin-9; SH3 and PX domain-containing protein 1; Protein SDP1; SH3 and PX domain-containing protein 3A; SNX9 |
| Background | Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is SH3PX1 Polyclonal Antibody, PE-Cy7 Conjugated (bs-12407R-PE-Cy7) validated for, and at what dilution?
bs-12407R-PE-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SH3PX1 Polyclonal Antibody, PE-Cy7 Conjugated (bs-12407R-PE-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse.
How should bs-12407R-PE-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SH3PX1 Polyclonal Antibody, PE-Cy7 Conjugated (bs-12407R-PE-Cy7)?
KLH conjugated synthetic peptide derived from human SNX9
What are the excitation and emission wavelengths of the PE-Cy7 conjugate (bs-12407R-PE-Cy7)?
PE-Cy7 on bs-12407R-PE-Cy7: excitation / emission 488nm/780nm.