CAPRIN2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12423R-Cy5-100uL |
| Product Name | CAPRIN2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CAPRIN2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 65981 |
| Swiss Prot | Q6IMN6 |
| Subcellular location | Cytoplasm |
| Synonyms | C1q domain-containing protein 1; C1QDC1; CAPR2_HUMAN; Caprin family member 2; Caprin-2; caprin2; Cytoplasmic activation/proliferation-associated protein 2; EEG 1; EEG1; Gastric cancer multidrug resistance-associated protein; KIAA1873; Protein EEG-1; RNA granule protein 140; RNG140. |
| Background | Caprin2 is 1127 amino acid highly conserved protein that is ubiquitously expressed with highest levels of expression in brain and spleen. Caprin2 stabilizes cytosolic _-catenin and enhances LEF-1 dependent reporter gene activity as well as the expression of Wnt target genes in mammalian cells. Caprin2 promotes LRP5/6 phosphorylation by GSK-3 and enhances the interaction between Axin and LRP5/6. It is suggested that Caprin2 functions as a proapoptotic inhibitor of the cell cycle. Nine isoforms of Caprin2 exist due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CAPRIN2 Polyclonal Antibody, Cy5 Conjugated (bs-12423R-Cy5) validated for, and at what dilution?
bs-12423R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CAPRIN2 Polyclonal Antibody, Cy5 Conjugated (bs-12423R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Sheep, Pig, Horse, Chicken.
How should bs-12423R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CAPRIN2 Polyclonal Antibody, Cy5 Conjugated (bs-12423R-Cy5)?
KLH conjugated synthetic peptide derived from human CAPRIN2
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12423R-Cy5)?
Cy5 on bs-12423R-Cy5: excitation / emission 625,650nm/670nm.