Caseine Kinase 1 alpha Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12427R-100uL |
| Product Name | Caseine Kinase 1 alpha Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will cross-react with the delta and epsilon isoforms. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CK1A/Caseine Kinase 1 alpha |
| Immunogen Range | 221-337/337 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1452 |
| Swiss Prot | P48729 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CK1; CK1a; CKIa; HLCDGP1; PRO2975; HEL-S-77p; Casein kinase I isoform alpha; CKI-alpha; CSNK1A1 |
| Background | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Caseine Kinase 1 alpha Antibody (bs-12427R) validated for, and at what dilution?
bs-12427R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caseine Kinase 1 alpha Antibody (bs-12427R) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep, Horse, Rabbit.
How should bs-12427R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caseine Kinase 1 alpha Antibody (bs-12427R)?
KLH conjugated synthetic peptide derived from human CK1A/Caseine Kinase 1 alpha Immunogen range: 221-337/337.