phospho-Caseine Kinase 1 alpha(Tyr294) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12428R-Cy5.5-100uL |
| Product Name | phospho-Caseine Kinase 1 alpha(Tyr294) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Sheep, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Casein Kinase 1 alpha around the phosphorylation site of Tyr294 |
| Modification Site | Tyr294 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1452 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Casein Kinase 1 alpha phospho Y294; p-CK1A phospho Y294; Casein kinase 1 alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI alpha; CKI-alpha; CKIa; Clock regulator kinase; Csnk1a1; Down regulated in lung cancer; HLCDGP1; KC1A_HUMAN; PRO2975. |
| Background | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Caseine Kinase 1 alpha(Tyr294) Polyclonal Antibody, Cy5.5 Conjugated (BS-12428R-CY5-5) validated for, and at what dilution?
BS-12428R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Caseine Kinase 1 alpha(Tyr294) Polyclonal Antibody, Cy5.5 Conjugated (BS-12428R-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Sheep, Chicken, Rabbit.
How should BS-12428R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Caseine Kinase 1 alpha(Tyr294) Polyclonal Antibody, Cy5.5 Conjugated (BS-12428R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human Casein Kinase 1 alpha around the phosphorylation site of Tyr294
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-12428R-CY5-5)?
Cy5.5 on BS-12428R-CY5-5: excitation / emission 675nm/694nm.