phospho-AR/Androgen receptor (Ser515) Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12471R-BF594-100uL |
| Product Name | phospho-AR/Androgen receptor (Ser515) Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser515 |
| Modification Site | Ser515 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 367 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Androgen Receptor phospho S515; Androgen Receptor Ser515; Androgen Receptor phospho Ser515; p-Androgen Receptor Ser515; ANDR_HUMAN; HYSP1; AIS; Androgen receptor dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease; AR; DHTR; Dihydro Testosterone Receptor; Dihydrotestosterone receptor; HUMARA; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; Spinal and bulbar muscular atrophy; TFM. |
| Background | The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-AR/Androgen receptor (Ser515) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12471R-BF594) validated for, and at what dilution?
bs-12471R-BF594 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-AR/Androgen receptor (Ser515) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12471R-BF594) react with?
Tested reactivity: Human.
How should bs-12471R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-AR/Androgen receptor (Ser515) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12471R-BF594)?
KLH conjugated synthetic phosphopeptide derived from human Androgen Receptor around the phosphorylation site of Ser515
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-12471R-BF594)?
AbBy Fluor-594 on bs-12471R-BF594: excitation / emission 590nm/617nm.