ANKK1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12475R-Cy3-100uL |
| Product Name | ANKK1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Horse, Chicken |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ANKK1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 255239 |
| Subcellular location | Cytoplasm |
| Synonyms | Ankyrin repeat and kinase domain containing 1; Ankyrin repeat and protein kinase domain containing protein 1; EC 2.7.11.1; PKK2; Protein kinase PKK2; SGK288; Sugen kinase 288; X kinase; ANKK1_HUMAN. |
| Background | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. ANKK1 (ankyrin repeat and kinase domain containing 1), also known as PKK2 or SGK288, is a 765 amino acid protein that belongs to the Ser/Thr protein kinase family and contains 12 ANK repeats and one protein kinase domain. Highly expressed in brain tissue and present in lower amounts in placenta and spinal cord, ANKK1 functions to catalyze the ATP-dependent phosphorylation of target proteins and is thought to play a role in alcohol and nicotine dependence. The gene encoding ANKK1 maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ANKK1 Polyclonal Antibody, Cy3 Conjugated (bs-12475R-Cy3) validated for, and at what dilution?
bs-12475R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ANKK1 Polyclonal Antibody, Cy3 Conjugated (bs-12475R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Horse, Chicken.
How should bs-12475R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ANKK1 Polyclonal Antibody, Cy3 Conjugated (bs-12475R-Cy3)?
KLH conjugated synthetic peptide derived from human ANKK1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-12475R-Cy3)?
Cy3 on bs-12475R-Cy3: excitation / emission 512,550nm/570,615nm.