APOA1BP/AI-BP Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12491R-Cy5.5-100uL |
| Product Name | APOA1BP/AI-BP Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Rabbit, Chimpanzee |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human APOA1BP/AI-BP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 128240 |
| Subcellular location | Secreted |
| Synonyms | AI BP; AIBP; ApoA I binding protein; Apolipoprotein A I binding protein; MGC119143; MGC119144; MGC119145; YjeF N terminal domain containing protein 1; YJEFN1; NNRE_HUMAN. |
| Background | Apolipoproteins are protein components of plasma lipoproteins. Apolipoprotein A-I (ApoA1) promotes cholesterol efflux from tissues to the liver for excretion. ApoA1 is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a cofactor for lecithin cholesterolacyltransferase, which is responsible for the formation of most plasma cholesteryl esters. AI-BP (Apolipoprotein A-I-binding protein), also known as YjeF N-terminal domain-containing protein 1, is a 288 amino acid secreted protein that binds ApoA1, ApoA2 and HDL. Individuals with impaired renal function show an increased rate of AI-BP excretion, indicating that it is normally reabsorbed within the kidney tubules. AI-BP belongs to the YjeF N-terminal domain protein family, which includes proteins that are frequently involved in oogenesis and spermatogenesis. There are two isoforms of AI-BP that are produced as a result of alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is APOA1BP/AI-BP Polyclonal Antibody, Cy5.5 Conjugated (BS-12491R-CY5-5) validated for, and at what dilution?
BS-12491R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APOA1BP/AI-BP Polyclonal Antibody, Cy5.5 Conjugated (BS-12491R-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Rabbit, Chimpanzee.
How should BS-12491R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APOA1BP/AI-BP Polyclonal Antibody, Cy5.5 Conjugated (BS-12491R-CY5-5)?
KLH conjugated synthetic peptide derived from human APOA1BP/AI-BP
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-12491R-CY5-5)?
Cy5.5 on BS-12491R-CY5-5: excitation / emission 675nm/694nm.