APOL1 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12498R-APC-Cy7 |
| Product Name | APOL1 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human APOL1/Apolipoprotein L |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8542 |
| Subcellular location | Secreted |
| Synonyms | APO L; Apo-L; APOL; APOL I; ApoL-I; APOL1; Apolipoprotein L1; APOL1_HUMAN; APOLI; APOL1; APOL 1; APOL-1; Apolipoprotein L; Apolipoprotein L I; Apolipoprotein L-I; Apolipoprotein L1; Apolipoprotein L-1; FSGS4. |
| Background | Apolipoproteins are protein components of plasma lipoproteins (1). The apolipoprotein L gene family encodes six highly homologous proteins designated apoL-I to -VI, which are associated with large high density type lipoproteins (HDL) (2,3). The human apoL family maps to chromosome 22q12.1-13.1 within a 127,000-bp region (4). ApoL has been characterized as a pancreas specific, 383-amino acid protein that contains a 12-amino acid secretory signal peptide (4). The apoL genes have TATA-less promoters and contain putative sterol regulatory elements, suggesting that transcription of these genes may be coordinated with that of the low density lipoprotein receptor and genes in pathways involving the synthesis of triglycerides and cholesterol (3). ApoL homologs can undergo 10 fold changes in expression during atherosclerotic changes in vascular endothelial cells, which includes the inflammatory reaction of atherosclerotic lesions (5). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is APOL1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12498R-APC-Cy7) validated for, and at what dilution?
bs-12498R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does APOL1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12498R-APC-Cy7) react with?
Tested reactivity: Human.
How should bs-12498R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APOL1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12498R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human APOL1/Apolipoprotein L
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-12498R-APC-Cy7)?
APC-Cy7 on bs-12498R-APC-Cy7: excitation / emission 650nm/780nm.