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APOL5 Polyclonal Antibody, AbBy Fluor-488 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
Overview
Catalog # bs-12500R-BF488
Product Name APOL5 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human APOL5/Apolipoprotein L5
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 80831
Subcellular location Cytoplasm
Synonyms APOL 5; APOL V; APOL5; Apolipoprotein L; Apolipoprotein L V; apoL-V; Apolipoprotein L 5; APOLV; OTTHUMP00000028773; APOL5_HUMAN.
Background The apolipoprotein L gene family maps to a region on chromosome 22 and encodes six highly homologous proteins designated apoL-I, apoL-II, apoL-III, apoL-IV, apoL-V and apoL-VI, all of which function as components of plasma lipoproteins. ApoL-V (apolipoprotein L-V), also known as APOL5, is a 433 amino acid protein that localizes to the cytoplasm and belongs to the apolipoprotein L family. Expressed in a variety of tissues including testis, stomach, uterus and skeletal muscle, apoL-V is thought to affect the movement of lipids in the cytoplasm and may allow the binding of lipids to organelles. Like other members of the apolipoprotein L family, apoL-V is thought to be involved in the development of schizophrenia.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is APOL5 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-12500R-BF488) validated for, and at what dilution?

bs-12500R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-12500R-BF488 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise APOL5 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-12500R-BF488)?

KLH conjugated synthetic peptide derived from human APOL5/Apolipoprotein L5

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-12500R-BF488)?

AbBy Fluor-488 on bs-12500R-BF488: excitation / emission 499nm/519nm.