APOL5 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12500R-Cy5.5-100uL |
| Product Name | APOL5 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human APOL5/Apolipoprotein L5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80831 |
| Subcellular location | Cytoplasm |
| Synonyms | APOL 5; APOL V; APOL5; Apolipoprotein L; Apolipoprotein L V; apoL-V; Apolipoprotein L 5; APOLV; OTTHUMP00000028773; APOL5_HUMAN. |
| Background | The apolipoprotein L gene family maps to a region on chromosome 22 and encodes six highly homologous proteins designated apoL-I, apoL-II, apoL-III, apoL-IV, apoL-V and apoL-VI, all of which function as components of plasma lipoproteins. ApoL-V (apolipoprotein L-V), also known as APOL5, is a 433 amino acid protein that localizes to the cytoplasm and belongs to the apolipoprotein L family. Expressed in a variety of tissues including testis, stomach, uterus and skeletal muscle, apoL-V is thought to affect the movement of lipids in the cytoplasm and may allow the binding of lipids to organelles. Like other members of the apolipoprotein L family, apoL-V is thought to be involved in the development of schizophrenia. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is APOL5 Polyclonal Antibody, Cy5.5 Conjugated (BS-12500R-CY5-5) validated for, and at what dilution?
BS-12500R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-12500R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise APOL5 Polyclonal Antibody, Cy5.5 Conjugated (BS-12500R-CY5-5)?
KLH conjugated synthetic peptide derived from human APOL5/Apolipoprotein L5
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-12500R-CY5-5)?
Cy5.5 on BS-12500R-CY5-5: excitation / emission 675nm/694nm.