Apolipoprotein O Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12502R-APC |
| Product Name | Apolipoprotein O Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ApoO/Apolipoprotein O |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79135 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | ApoO; 0610008C08Rik; 1110019O03Rik; Apolipoprotein O; APOO; APOO_HUMAN; FAM121B; Family with sequence similarity 121B; MGC130105; MGC130106; MGC4825; My025; MYO25; OTTHUMP00000023073; Protein FAM121B; RP23-272D10.2. |
| Background | Apolipoproteins are a family of fatty-acid binding proteins that transport fat through the bloodstream in the form of lipoproteins. ApoO (Apolipoprotein O), also known as FAM121B or My025, is a 198 amino acid single-pass membrane protein that belongs to the apolipoprotein family. Expressed ubiquitously with particularly high expression in diabetic heart tissue, apoO functions to promote the transport of cholesterol from macrophage cells and may be involved in regulatory mechanisms that protect lipid accumulation within the heart. ApoO is present in high density lipoproteins (HDLs) and low density lipoproteins (LDLs) and is secreted by an MTP (microsomal triglyceride transfer protein)-dependent mechanism. Two isoforms of apoO exist due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Apolipoprotein O Polyclonal Antibody, APC Conjugated (bs-12502R-APC) validated for, and at what dilution?
bs-12502R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Apolipoprotein O Polyclonal Antibody, APC Conjugated (bs-12502R-APC) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-12502R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Apolipoprotein O Polyclonal Antibody, APC Conjugated (bs-12502R-APC)?
KLH conjugated synthetic peptide derived from human ApoO/Apolipoprotein O
What are the excitation and emission wavelengths of the APC conjugate (bs-12502R-APC)?
APC on bs-12502R-APC: excitation / emission 650nm/660nm.