ARFGAP3 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12513R-BF647 |
| Product Name | ARFGAP3 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ARFGAP3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26286 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ADP ribosylation factor GTPase activating protein 1; ADP ribosylation factor GTPase activating protein 3; ADP-ribosylation factor GTPase-activating protein 3; ARF GAP 3; ARFG3_HUMAN; ArfGAP 3; ARFGAP1; ARFGAP3; FLJ45618. |
| Background | ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1), ARFGAP2 (ADP-ribosylation factor GTPase-activating protein 2) and ARFGAP3 (ADP-ribosylation factor GTPase-activating protein 3) are GTPase-activating proteins (GAP) that are associated with the Golgi apparatus and interact with ADP-ribosylation factor 1 (ARF). These proteins promote hydrolysis of ARF-bound GTP and are required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of ARFGAP1, ARFGAP2 and ARFGAP3 is stimulated by phosphoinosides and inhibited by phosphatidylcholine. The genes encoding ARFGAP1, ARFGAP2 and ARFGAP3 map to human chromosomes 20q13.33, 11p11.2 and 22q13.2, respectively. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ARFGAP3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12513R-BF647) validated for, and at what dilution?
bs-12513R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ARFGAP3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12513R-BF647) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Chicken.
How should bs-12513R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ARFGAP3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12513R-BF647)?
KLH conjugated synthetic peptide derived from human ARFGAP3
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-12513R-BF647)?
AbBy Fluor-647 on bs-12513R-BF647: excitation / emission 650nm/665nm.