Argininosuccinate Lyase Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12515R-BF405 |
| Product Name | Argininosuccinate Lyase Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ASL/Argininosuccinate Lyase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 435 |
| Subcellular location | Cytoplasm, Extracellular matrix |
| Synonyms | Argininosuccinase; Argininosuccinate lyase; Arginosuccinase; ARLY_HUMAN; ASAL; ASL; EC 4.3.2.1. |
| Background | ASL is a member of the lyase 1 family of proteins and is predominantly expressed in the liver. Localizing to the cytoplasm and existing as a homotetramer, ASL catalyzes the hydrolytic cleavage of argininosuccinic acid (ASA) to fumarate and arginine, an essential step of the urea cycle which is crucial for the detoxification of ammonia. This reaction is also involved in the biosynthesis of arginine. In addition, ASL shares high sequence homology with the avian and reptilian eye lens protein, d-crystallin. Mutations in the gene encoding ASL leads to an accumulation of ASA in body fluids and results in Arginosuc-cinic aciduria (ASAuria), an autosomal recessive disorder that is characterized by hyperammonemia, liver enlargement, convulsions, physical and mental retardation, episodic unconsciousness and dry and brittle hair showing trich-orrhexis nodosa (weak points or nodes in the hair shaft). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Argininosuccinate Lyase Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-12515R-BF405) validated for, and at what dilution?
bs-12515R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12515R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Argininosuccinate Lyase Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-12515R-BF405)?
KLH conjugated synthetic peptide derived from human ASL/Argininosuccinate Lyase
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-12515R-BF405)?
AbBy Fluor-405 on bs-12515R-BF405: excitation / emission 401nm/421nm.