ARL4 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12523R-Cy5.5-100uL |
| Product Name | ARL4 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ARL4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10124 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | ADP ribosylation factor like 4A; ADP-ribosylation factor-like protein 4A; ARL 4; ARL4A; ARL4A_HUMAN. |
| Background | ADP-ribosylation factors (ARFs) are highly conserved guanine nucleotide-binding proteins that enhance the ADP-ribosyltransferase activity of cholera toxin. ARF_ are important in eukaryotic vesicular trafficking pathways and activating phospholipase D. ARL4 (ADP-ribosylation factor-like protein 4A) is a member of the ARF-like protein (ARL) subfamily of small GTPases. It contains a C terminal nuclear localization signal (NLS) region that interacts with Importin-? ARL4 localizes to the nucleus and is found in a variety of tissues, but is predominantly expressed in spermatogonia and Sertoli cells. It is most closely related to ARL6 and ARL7. Unlike ARFs, ARL4 does not activate the cholera toxin ADP-ribosyltranferase. ARL4 may play a role in neurogenesis during embryonic development and somitogenesis in the early stages of adult spermatogenesis. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ARL4 Polyclonal Antibody, Cy5.5 Conjugated (BS-12523R-CY5-5) validated for, and at what dilution?
BS-12523R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-12523R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ARL4 Polyclonal Antibody, Cy5.5 Conjugated (BS-12523R-CY5-5)?
KLH conjugated synthetic peptide derived from human ARL4
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-12523R-CY5-5)?
Cy5.5 on BS-12523R-CY5-5: excitation / emission 675nm/694nm.