ARPC2 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12527R-RBITC |
| Product Name | ARPC2 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ARPC2/p34-ARC |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10109 |
| Swiss Prot | O15144 |
| Subcellular location | Cytoplasm |
| Synonyms | ARC34; PRO2446; p34-Arc; PNAS-139; Actin-related protein 2/3 complex subunit 2; Arp2/3 complex 34 kDa subunit; ARPC2 |
| Background | Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ARPC2 Polyclonal Antibody, RBITC Conjugated (bs-12527R-RBITC) validated for, and at what dilution?
bs-12527R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ARPC2 Polyclonal Antibody, RBITC Conjugated (bs-12527R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig.
How should bs-12527R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ARPC2 Polyclonal Antibody, RBITC Conjugated (bs-12527R-RBITC)?
KLH conjugated synthetic peptide derived from human ARPC2/p34-ARC
What are the excitation and emission wavelengths of the RBITC conjugate (bs-12527R-RBITC)?
RBITC on bs-12527R-RBITC: excitation / emission 540nm/625nm.