API5 Polyclonal Antibody

$Lane A: Mouse Heart lysate Lane B: Mouse Pancreas lysates probed with Rabbit Anti-API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:300 overnight at 4˚C. Followed by HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 for 90 min at 37˚C. \\n$

Lane A: Mouse Heart lysate Lane B: Mouse Pancreas lysates probed with Rabbit Anti-API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:300 overnight at 4˚C. Followed by HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1:5000 for 90 min at 37˚C. \\n

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with API5 Polyclonal Antibody, Unconjugated (bs-1252R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Pig
Horse
Guinea Pig

Overview
Catalog #	bs-1252R
Product Name	API5 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Pig, Horse, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human API5
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8539
Swiss Prot	Q9BZZ5
Subcellular location	Cytoplasm, Nucleus
Synonyms	AAC11; AAC-11; Apoptosis inhibitor 5; API-5; Antiapoptosis clone 11 protein; Cell migration-inducing gene 8 protein; Fibroblast growth factor 2-interacting factor; FIF; Protein XAGL; API5; MIG8
Background	Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Application Dilution
WB	1:300-5000
IHC-P	ELISA1:500-1000
IHC-F	FCM1:20-100
IF