ATF5 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12542R-FITC |
| Product Name | ATF5 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATF5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22809 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Activating transcription factor 5 alpha/beta; Activating transcription factor 5; Activating transcription factor X; AFTA; ATF 5; ATF 7; ATF7; ATFX; BZIP protein ATF7; cAMP dependent transcription factor ATF 5; cAMP dependent transcription factor ATF5; Cyclic AMP dependent transcription factor ATF 5; Cyclic AMP dependent transcription factor ATF5; FLJ34666; HMFN0395; MGC102397; NAP1; NR3 associated protein; ODA 10; Transcription factor ATFx; Transcription factor like protein ODA 10; Transcription factor like protein ODA10; ATF5_HUMAN. |
| Background | Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB3 and Luman), CREM-1, CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-5 (ATFx), which can localize to the cytoplasm or the nucleus, binds DNA as a dimer. It interacts with CCND3 and PTP4A1. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ATF5 Polyclonal Antibody, FITC Conjugated (bs-12542R-FITC) validated for, and at what dilution?
bs-12542R-FITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATF5 Polyclonal Antibody, FITC Conjugated (bs-12542R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse.
How should bs-12542R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATF5 Polyclonal Antibody, FITC Conjugated (bs-12542R-FITC)?
KLH conjugated synthetic peptide derived from human ATF5
What are the excitation and emission wavelengths of the FITC conjugate (bs-12542R-FITC)?
FITC on bs-12542R-FITC: excitation / emission 494nm/518nm.