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ATF6 beta Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-12543R-Cy5.5-100uL
Product Name ATF6 beta Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ATF6 beta
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1388
Swiss Prot Q99941
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms G13; CREBL1; CREB-RP; Cyclic AMP-dependent transcription factor ATF-6 beta; cAMP-dependent transcription factor ATF-6 beta; Activating transcription factor 6 beta; ATF6-beta; Protein G13; cAMP response element-binding protein-related protein; cAMP-responsive element-binding protein-like 1; ATF6B
Background Transcriptional factor that acts in the unfolded protein response (UPR) pathway by activating UPR target genes induced during ER stress. Binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAATN(9)CCAC[GA]-3') when NF-Y is bound to ERSE.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ATF6 beta Polyclonal Antibody, Cy5.5 Conjugated (BS-12543R-CY5-5) validated for, and at what dilution?

BS-12543R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ATF6 beta Polyclonal Antibody, Cy5.5 Conjugated (BS-12543R-CY5-5) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Rabbit.

How should BS-12543R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ATF6 beta Polyclonal Antibody, Cy5.5 Conjugated (BS-12543R-CY5-5)?

KLH conjugated synthetic peptide derived from human ATF6 beta

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-12543R-CY5-5)?

Cy5.5 on BS-12543R-CY5-5: excitation / emission 675nm/694nm.

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