Help

ATP6V0D2 Polyclonal Antibody, APC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Sheep
  • Pig
Overview
Catalog # bs-12548R-APC
Product Name ATP6V0D2 Polyclonal Antibody, APC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Sheep, Pig
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ATP6V0D2/V-ATPase D2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 245972
Swiss Prot Q8N8Y2
Subcellular location Cytoplasm, Cell membrane
Synonyms VMA6; ATP6D2; V-type proton ATPase subunit d 2; V-ATPase subunit d 2; Vacuolar proton pump subunit d 2; ATP6V0D2
Background Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity).
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ATP6V0D2 Polyclonal Antibody, APC Conjugated (bs-12548R-APC) validated for, and at what dilution?

bs-12548R-APC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ATP6V0D2 Polyclonal Antibody, APC Conjugated (bs-12548R-APC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Sheep, Pig.

How should bs-12548R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ATP6V0D2 Polyclonal Antibody, APC Conjugated (bs-12548R-APC)?

KLH conjugated synthetic peptide derived from human ATP6V0D2/V-ATPase D2

What are the excitation and emission wavelengths of the APC conjugate (bs-12548R-APC)?

APC on bs-12548R-APC: excitation / emission 650nm/660nm.