ATP6V0D2 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12548R-APC-Cy7 |
| Product Name | ATP6V0D2 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Sheep, Pig |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATP6V0D2/V-ATPase D2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 245972 |
| Swiss Prot | Q8N8Y2 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | VMA6; ATP6D2; V-type proton ATPase subunit d 2; V-ATPase subunit d 2; Vacuolar proton pump subunit d 2; ATP6V0D2 |
| Background | Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis (By similarity). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ATP6V0D2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12548R-APC-Cy7) validated for, and at what dilution?
bs-12548R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATP6V0D2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12548R-APC-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Sheep, Pig.
How should bs-12548R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATP6V0D2 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12548R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human ATP6V0D2/V-ATPase D2
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-12548R-APC-Cy7)?
APC-Cy7 on bs-12548R-APC-Cy7: excitation / emission 650nm/780nm.