ATPIF1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12550R-BF680 |
| Product Name | ATPIF1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATPIF1/ATPase Inhibitory Factor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 93974 |
| Subcellular location | Cytoplasm |
| Synonyms | AT1_HUMAN; ATP synthase inhibitor protein; ATPase inhibitor; ATPase inhibitor mitochondrial; ATPase inhibitor protein; ATPase inhibitory factor 1; ATPI; ATP 1; ATP1; ATPIP; 1; 1; Inhibitor of F1Fo-ATPase; IP; MGC1167; MGC8898; mitochondrial. |
| Background | Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three Alpha chains and three Beta chains, which are weakly homologous, as well as one Gamma chain, one Delta chain and one Gamma chain. F0 consists of three subunits: a, b and c. A mitochondrial F1-ATPase inhibitor protein, ATPIF1 (ATPase inhibitory factor 1), also known as IP, IF1, ATPI or ATPIP (ATPase inhibitor protein), binds to the C-terminal region of a Beta subunit of the F1-ATPase at low pH values and, via interference of the Beta and Gamma subunit interaction, ATPIF1 regulates the activity of the F1F0-ATPase. This reversible ATPIF1 binding to F1F0-ATPase also occurs on the surface of endothelial cells. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is ATPIF1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12550R-BF680) validated for, and at what dilution?
bs-12550R-BF680 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATPIF1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12550R-BF680) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Horse, Rabbit.
How should bs-12550R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATPIF1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-12550R-BF680)?
KLH conjugated synthetic peptide derived from human ATPIF1/ATPase Inhibitory Factor 1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-12550R-BF680)?
AbBy Fluor-680 on bs-12550R-BF680: excitation / emission 679nm/702nm.