phospho-BRAF (Thr598) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12557R-APC-100uL |
| Product Name | phospho-BRAF (Thr598) Polyclonal Antibody, APC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Thr599 |
| Modification Site | Thr599 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 673 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1; RAFB1 |
| Background | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-BRAF (Thr598) Polyclonal Antibody, APC Conjugated (bs-12557R-APC) validated for, and at what dilution?
bs-12557R-APC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-BRAF (Thr598) Polyclonal Antibody, APC Conjugated (bs-12557R-APC) react with?
Tested reactivity: Human.
How should bs-12557R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-BRAF (Thr598) Polyclonal Antibody, APC Conjugated (bs-12557R-APC)?
KLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Thr599
What are the excitation and emission wavelengths of the APC conjugate (bs-12557R-APC)?
APC on bs-12557R-APC: excitation / emission 650nm/660nm.