B7-H6 Polyclonal Antibody

Lane 1:Huh7 cell lysate Lane 2: A549 cell lysates probed with Rabbit Anti-B7-H6 Polyclonal Antibody, Unconjugated (bs-12559R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 15000 for 90 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with B7-H6 Polyclonal Antibody, Unconjugated (bs-12559R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with B7-H6 Polyclonal Antibody, Unconjugated (bs-12559R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse

Overview
Catalog #	bs-12559R
Product Name	B7-H6 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human B7-H6
Immunogen Range	51-150/454
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	374383
Swiss Prot	Q68D85
Subcellular location	Cell membrane
Synonyms	B7H6; B7-H6; DKFZp686O24166; Natural cytotoxicity triggering receptor 3 ligand 1; B7 homolog 6; NCR3LG1
Background	Triggers NCR3-dependent natural killer cell activation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

Applications

Reactivity

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