phospho-Band3 (Tyr21) Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12569R-BF488 |
| Product Name | phospho-Band3 (Tyr21) Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Dog |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Band3 around the phosphorylation site of Tyr21 |
| Modification Site | Tyr21 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6521 |
| Swiss Prot | P02730 |
| Subcellular location | Cell membrane |
| Synonyms | SLC4A1; Band 3 phospho Y21; p-Band 3 phospho Y21; Solute carrier family 4 anion exchanger member 1; Solute carrier family 4 member 1; AE 1; AE1; Anion exchange protein 1; Anion exchanger 1; B3AT_HUMAN; Band 3; Band 3 anion transport protein; BND3; CD233; DI; Diego blood group; EMPB3; EPB3; Erythrocyte membrane protein band 3; Erythroid anion exchange protein; FR antibod; Froese blood group; RTA1A; Solute carrier family 4 member 1; SW antibody; Swann blood group; Waldner blood group; WD antibody; WD1; WR antibody; Wright blood group. |
| Background | Band 3, also designated AE1, is an erythrocyte membrane glycoprotein that contributes to cell stuctural integrity and mediates exchange of chloride and bicarbonate across the phospholipid bilayer. The diverse functions of the approximately 900 amino acid protein are mediated by two distinct domains. The amino terminal domain, also known as cdb3 for cytoplasmic domain of erthrocyte membrane band 3, acts as an attachment site for the erythrocyte skeleton by binding ankyrin. The carboxy-terminal, membrane-associated domain carries out exchange transport of anions. Degradation of band 3 can generate an aging antigen known as senescent cell antigen, or SCA, which is expressed on old cells and marks them for removal by the immune system. An isoform of band 3, which lacks the first 65 amino acids and does not bind ankryin, is expressed in kidney. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Band3 (Tyr21) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-12569R-BF488) validated for, and at what dilution?
bs-12569R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12569R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Band3 (Tyr21) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-12569R-BF488)?
KLH conjugated synthetic phosphopeptide derived from human Band3 around the phosphorylation site of Tyr21
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-12569R-BF488)?
AbBy Fluor-488 on bs-12569R-BF488: excitation / emission 499nm/519nm.