BANF1 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12571R-PE-Cy5 |
| Product Name | BANF1 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BANF1/BAF |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8815 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | BAF; BAF_HUMAN; BANF 1; BANF1; Barrier to autointegration factor 1; Barrier to autointegration factor; Barrier-to-autointegration factor; BCRG 1; BCRG1; BCRP 1; BCRP1; Breakpoint cluster region protein 1; D14S1460; MGC111161. |
| Background | Barrier-to-autointegration factor (BAF) binds non-specifically to double stranded DNA, possibly to play a role in tissue- or cell type-specific gene expression by interacting with different homeodomain transcription factors. BAF compresses chromatin structure and interacts with the LEM domain of nuclear proteins to play a crucial role in membrane recruitment and chromatin decondensation during nuclear assembly. Additionally, retroviruses like HIV-1 incorporate BAF from host cells into preintegration complexes (PICs) to prevent autointegration of retroviral DNA and thereby promote integration of retroviral DNA into the host chromosome. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is BANF1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-12571R-PE-Cy5) validated for, and at what dilution?
bs-12571R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12571R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BANF1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-12571R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human BANF1/BAF
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-12571R-PE-Cy5)?
PE-Cy5 on bs-12571R-PE-Cy5: excitation / emission 488nm/670nm.