beta B1 Crystallin Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12582R-100uL |
| Product Name | beta B1 Crystallin Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human beta B1 Crystallin |
| Immunogen Range | 101-200/252 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1414 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Beta crystallin B1; Beta-B1 crystallin; Beta-crystallin B1; CRBB1_HUMAN; CRYBB 1; Crybb1; Crystallin beta B1; Eye lens structural protein; OTTHUMP00000028719. |
| Background | Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into Alpha, Beta, and Gamma families, and the Beta- and Gamma-crystallins also comprise a superfamily. Crystallins usually contain seven distinctive protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins constitute the major lens structural proteins, and they associate into dimers, tetramers, and higher order aggregates. The Beta-crystallin subfamily is composed of several gene products, including Beta A1-, Beta A2-, Beta A3-, Beta A4-, Beta B1-, Beta B2- and Beta B3-crystallin. The Beta A1- and Beta A3-crystallin proteins are encoded by a single mRNA. They differ by only 17 amino acids, and Beta A1-crystallin is generated by use of an alternate translation initiation site. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is beta B1 Crystallin Polyclonal Antibody (bs-12582R) validated for, and at what dilution?
bs-12582R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does beta B1 Crystallin Polyclonal Antibody (bs-12582R) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Sheep, Pig, Rabbit.
How should bs-12582R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise beta B1 Crystallin Polyclonal Antibody (bs-12582R)?
KLH conjugated synthetic peptide derived from human beta B1 Crystallin Immunogen range: 101-200/252.