phospho-PDCD4 (Ser457) Polyclonal Antibody

$HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-PDCD4 (Ser457)) polyclonal Antibody, Unconjugated (bs-12591R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-PDCD4 (Ser457)) polyclonal Antibody, Unconjugated (bs-12591R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ICC/IF

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Sheep
Pig
Horse
Chicken
Rabbit
Zebrafish

Overview
Catalog #	bs-12591r
Product Name	phospho-PDCD4 (Ser457) Polyclonal Antibody
Applications	WB, ICC/IF
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthesised phosphopeptide derived from human PDCD4 around the phosphorylation site of Ser457
Modification Site	Ser457
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	27250
Swiss Prot	Q53EL6
Subcellular location	Cytoplasm, Nucleus
Synonyms	PDCD4 (phospho S457); p-PDCD4 (phospho S457); Death up-regulated gene protein; Dug; H731; Ma3; MGC33046; MGC33047; Neoplastic transformation inhibitor; Neoplastic transformation inhibitor protein; Nuclear antigen H731; Nuclear antigen H731 like; Nuclear antigen H731 like protein; Nuclear antigen H731-like; PDCD 4; Pdcd4; PDCD4_HUMAN; Programmed cell death 4; programmed cell death 4 (neoplastic transformation inhibitor); Programmed cell death protein 4; Protein 197/15a; Protein MA-3; Tis; Topoisomerase-inhibitor suppressed protein.
Application Dilution
WB	=1:500-2000
ICC/IF	ICC/IF=1:100