PELI3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12613R-PerCP-Cy5.5 |
| Product Name | PELI3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PELI3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 246330 |
| Swiss Prot | Q8N2H9 |
| Subcellular location | Cytoplasm |
| Synonyms | E3 ubiquitin-protein ligase pellino homolog 3; PELI3; Pellino-3; RING-type E3 ubiquitin transferase pellino homolog 3 |
| Background | E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1. Can activate AP1/JUN and ELK1. Not required for NF-kappa-B activation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PELI3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-12613R-PERCP-CY5-5) validated for, and at what dilution?
BS-12613R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PELI3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-12613R-PERCP-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should BS-12613R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PELI3 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-12613R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human PELI3
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-12613R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-12613R-PERCP-CY5-5: excitation / emission 482nm/694nm.