SMPD4 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12641R-Cy5 |
| Product Name | SMPD4 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SMPD4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55627 |
| Swiss Prot | Q9NXE4 |
| Subcellular location | Cytoplasm |
| Synonyms | EC 3.1.4.12; EC=3.1.4.123; FLJ20297; FLJ20756; KIAA1418; NET 13; NET13; Neutral sphingomyelinase 3; Neutral sphingomyelinase III; NSMA3_HUMAN; nSMase 3; nSMase-3; nSMase3; OTTHUMP00000162391; OTTHUMP00000190862; SKNY protein; smpd4; Sphingomyelin phosphodiesterase 4; Sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase 3). |
| Background | Catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SMPD4 Polyclonal Antibody, Cy5 Conjugated (bs-12641R-Cy5) validated for, and at what dilution?
bs-12641R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMPD4 Polyclonal Antibody, Cy5 Conjugated (bs-12641R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-12641R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMPD4 Polyclonal Antibody, Cy5 Conjugated (bs-12641R-Cy5)?
KLH conjugated synthetic peptide derived from human SMPD4
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12641R-Cy5)?
Cy5 on bs-12641R-Cy5: excitation / emission 625,650nm/670nm.