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SMARCA5 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
  • Zebrafish
  • GuineaPig
Overview
Catalog # bs-12653R-100uL
Product Name SMARCA5 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity Possible cross-reactivity with SMARCA1/SNF2L1
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish, GuineaPig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SNF2H
Immunogen Range 901-1000/1052
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 8476
Swiss Prot O60264
Subcellular location Nucleus
Synonyms 4933427E24Rik; ATP-dependent chromatin remodeling protein SNF2H; D030040M08Rik; D330027N15Rik; EC 3.6.1.-; hISWI; hSNF2H; ISWI; MommeD4; SMARCA5; SMCA5; SMCA5_HUMAN; Snf2h; Sucrose nonfermenting like 5; Sucrose nonfermenting protein 2 homolog; Sucrose nonfermenting, yeast, homolog of; sucrose nonfermenting-like 5; SWI/SNF related matrix associated actin dependent regulator of chromatin a5; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 5; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; WCRF135.
Background The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SMARCA5 Polyclonal Antibody (bs-12653R) validated for, and at what dilution?

bs-12653R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SMARCA5 Polyclonal Antibody (bs-12653R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish, GuineaPig.

How should bs-12653R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SMARCA5 Polyclonal Antibody (bs-12653R)?

KLH conjugated synthetic peptide derived from human SNF2H Immunogen range: 901-1000/1052.

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