SMARCA5 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12653R-BF647-100uL |
| Product Name | SMARCA5 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Possible cross-reactivity with SMARCA1/SNF2L1 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SNF2H |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8476 |
| Swiss Prot | O60264 |
| Subcellular location | Nucleus |
| Synonyms | 4933427E24Rik; ATP-dependent chromatin remodeling protein SNF2H; D030040M08Rik; D330027N15Rik; EC 3.6.1.-; hISWI; hSNF2H; ISWI; MommeD4; SMARCA5; SMCA5; SMCA5_HUMAN; Snf2h; Sucrose nonfermenting like 5; Sucrose nonfermenting protein 2 homolog; Sucrose nonfermenting, yeast, homolog of; sucrose nonfermenting-like 5; SWI/SNF related matrix associated actin dependent regulator of chromatin a5; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 5; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5; WCRF135. |
| Background | The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SMARCA5 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12653R-BF647) validated for, and at what dilution?
bs-12653R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMARCA5 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12653R-BF647) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish, GuineaPig.
How should bs-12653R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMARCA5 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12653R-BF647)?
KLH conjugated synthetic peptide derived from human SNF2H
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-12653R-BF647)?
AbBy Fluor-647 on bs-12653R-BF647: excitation / emission 650nm/665nm.