VAC14 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12679R-Cy5-100uL |
| Product Name | VAC14 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(ICC)
IF
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VAC14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55697 |
| Swiss Prot | Q08AM6 |
| Synonyms | ArPIKfyve; FLJ10305; FLJ36622; FLJ46582; MGC149815; MGC149816; OTTHUMP00000174908; Protein VAC14 homolog; Tax1 (human T cell leukemia virus type I) binding protein 1; Tax1 (human T cell leukemia virus type I) binding protein 2; Tax1 binding protein 2; Tax1-binding protein 2; TAX1BP2; TRX; VAC 14; vac14; Vac14 homolog (S. cerevisiae); Vac14 homolog; VAC14_HUMAN. |
| Background | The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. VAC14 is a component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PtdIns(3,5)P2-synthesizing enzyme PIKFYVE (MIM 609414) and the PtdIns(3,5)P2 phosphatase FIG4 (MIM 609390). VAC14 functions as an activator of PIKFYVE (Sbrissa et al., 2007 [PubMed 17556371]).[supplied by OMIM, Feb 2010] |
| Application Dilution | |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is VAC14 Polyclonal Antibody, Cy5 Conjugated (bs-12679R-Cy5) validated for, and at what dilution?
bs-12679R-Cy5 is validated for IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VAC14 Polyclonal Antibody, Cy5 Conjugated (bs-12679R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, Zebrafish.
How should bs-12679R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VAC14 Polyclonal Antibody, Cy5 Conjugated (bs-12679R-Cy5)?
KLH conjugated synthetic peptide derived from human VAC14
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12679R-Cy5)?
Cy5 on bs-12679R-Cy5: excitation / emission 625,650nm/670nm.