phospho-DNM1L (Ser616) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12702R-Cy3 |
| Product Name | phospho-DNM1L (Ser616) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Pig, GuineaPig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human DRP1 around the phosphorylation site of Ser616 |
| Modification Site | Ser616 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10059 |
| Swiss Prot | O00429 |
| Subcellular location | Cytoplasm |
| Synonyms | DRP1 (phospho S616); p-DRP1 (phospho S616); P-DRP1 (Ser616); DLP1; dnm1l; DNM1L_HUMAN; Dnm1p/Vps1p-like protein; DVLP; Dymple; Dynamin 1 like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin like protein; Dynamin related protein 1; Dynamin-1-like protein; Dynamin-like protein 4; Dynamin-like protein; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV 11; FLJ41912; HdynIV; VPS1. |
| Background | This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-DNM1L (Ser616) Polyclonal Antibody, Cy3 Conjugated (bs-12702R-Cy3) validated for, and at what dilution?
bs-12702R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-DNM1L (Ser616) Polyclonal Antibody, Cy3 Conjugated (bs-12702R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Pig, GuineaPig.
How should bs-12702R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-DNM1L (Ser616) Polyclonal Antibody, Cy3 Conjugated (bs-12702R-Cy3)?
KLH conjugated synthesised phosphopeptide derived from human DRP1 around the phosphorylation site of Ser616
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-12702R-Cy3)?
Cy3 on bs-12702R-Cy3: excitation / emission 512,550nm/570,615nm.