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CNTF Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat
  • Rabbit

Predicted Reactivity

  • Mouse
  • Pig
Overview
Catalog # bs-1272R-RBITC
Product Name CNTF Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity Belongs to the CNTF family
Reactivity Human, Rat, Rabbit
Predicted Reactivity Mouse, Pig
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CNTF
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1270
Swiss Prot P26441
Subcellular location Cytoplasm
Synonyms HCNTF; Ciliary neurotrophic factor; CNTF
Background CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CNTF Polyclonal Antibody, RBITC Conjugated (bs-1272R-RBITC) validated for, and at what dilution?

bs-1272R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CNTF Polyclonal Antibody, RBITC Conjugated (bs-1272R-RBITC) react with?

Tested reactivity: Human, Rat, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig.

How should bs-1272R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CNTF Polyclonal Antibody, RBITC Conjugated (bs-1272R-RBITC)?

KLH conjugated synthetic peptide derived from human CNTF

What are the excitation and emission wavelengths of the RBITC conjugate (bs-1272R-RBITC)?

RBITC on bs-1272R-RBITC: excitation / emission 540nm/625nm.