PLVAP Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PLVAP Polyclonal Antibody, Unconjugated (bs-12737R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PLVAP Polyclonal Antibody, Unconjugated (bs-12737R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Lane 1: Mouse Prostate tissue lysates; Lane 2: Mouse Large intestine tissue lysates probed with PLVAP Polyclonal Antibody, Unconjugated (bs-12737R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Prostate tissue lysates; Lane 2: Mouse Large intestine tissue lysates probed with PLVAP Polyclonal Antibody, Unconjugated (bs-12737R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-12737R
Product Name	PLVAP Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PLVAP
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	83483
Swiss Prot	Q9BX97
Synonyms	FELS; Fenestrated endothelial linked structure protein; Fenestrated endothelial-linked structure protein; gp68; Hypothetical protein DKFZp667O074; Meca 32 antigen; MECA32 antigen; Panendothelial cell antigen; Plasmalemma vesicle associated protein; Plasmalemma vesicle protein 1; Plasmalemma vesicle-associated protein; Plvap; PLVAP_HUMAN; PV 1; PV-1; PV1; PV1 protein.
Background	Involved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability.
Application Dilution
WB	1:500-2000
IHC-P
IHC-F
IF