POLDIP2 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12756R-Cy5 |
| Product Name | POLDIP2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human POLDIP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26073 |
| Swiss Prot | Q9Y2S7 |
| Subcellular location | Nucleus |
| Synonyms | 38 kDa DNA polymerase delta interaction protein; delta interacting protein 2; DNA polymerase delta interaction protein p38; p38; PDIP 2; PDIP 38; PDIP2; PDIP2_HUMAN; PDIP38; POLD 4; POLD4; Poldip2; Polymerase (DNA directed) delta interacting protein 2; Polymerase delta interacting protein 38; Polymerase delta-interacting protein 2. |
| Background | This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit, as well as with proliferating cell nuclear antigen. The encoded protein maybe play a role in the ability of the replication fork to bypass DNA lesions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is POLDIP2 Polyclonal Antibody, Cy5 Conjugated (bs-12756R-Cy5) validated for, and at what dilution?
bs-12756R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does POLDIP2 Polyclonal Antibody, Cy5 Conjugated (bs-12756R-Cy5) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse.
How should bs-12756R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise POLDIP2 Polyclonal Antibody, Cy5 Conjugated (bs-12756R-Cy5)?
KLH conjugated synthetic peptide derived from human POLDIP2
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12756R-Cy5)?
Cy5 on bs-12756R-Cy5: excitation / emission 625,650nm/670nm.