VPS29 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12770R-BF750-100uL |
| Product Name | VPS29 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | 91% homology with Rat VPS29 Protein |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VPS29 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51699 |
| Swiss Prot | Q9UBQ0 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | DC15; DC7; DKFZp564F0223; FLJ20492; hVPS29; PEP11; PEP11 homolog; Retromer protein; Vacuolar protein sorting 29; Vacuolar protein sorting 29 (yeast homolog); Vacuolar protein sorting 29 (yeast); Vacuolar protein sorting 29 homolog (S. cerevisiae); Vacuolar protein sorting 29 homolog; Vacuolar protein sorting associated protein 29; Vacuolar protein sorting-associated protein 29; Vacuolar sorting protein VPS29/PEP11; Vesicle protein sorting 29; VPS 29; VPS29; VPS29_HUMAN; x 007 protein. |
| Background | This gene belongs to a group of vacuolar protein sorting (VPS) genes that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. The protein encoded by this gene is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. This VPS protein may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Alternative splice variants encoding different isoforms and representing non-protein coding transcripts have been found for this gene. [provided by RefSeq, Aug 2013] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is VPS29 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12770R-BF750) validated for, and at what dilution?
bs-12770R-BF750 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VPS29 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12770R-BF750) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish.
How should bs-12770R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VPS29 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-12770R-BF750)?
KLH conjugated synthetic peptide derived from human VPS29
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-12770R-BF750)?
AbBy Fluor-750 on bs-12770R-BF750: excitation / emission 749nm/775nm.