VPS45A Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12777R-Cy5.5 |
| Product Name | VPS45A Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VPS45A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11311 |
| Swiss Prot | Q9NRW7 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | h-VPS45; H1; H1VPS45; HGNC:14579; hlVps45; Leucocyte vacuolar protein sorting 45; OTTHUMP00000014482; Vacuolar protein sorting 45 homolog (S. cerevisiae); Vacuolar protein sorting 45A (yeast homolog); Vacuolar protein sorting 45A; Vacuolar protein sorting 45B (yeast); Vacuolar protein sorting-associated protein 45; VPS45; VPS45_HUMAN; VPS45A; VPS45B; VPS54A; VSP45; VSP45A. |
| Background | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2013] |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is VPS45A Polyclonal Antibody, Cy5.5 Conjugated (BS-12777R-CY5-5) validated for, and at what dilution?
BS-12777R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VPS45A Polyclonal Antibody, Cy5.5 Conjugated (BS-12777R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse.
How should BS-12777R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VPS45A Polyclonal Antibody, Cy5.5 Conjugated (BS-12777R-CY5-5)?
KLH conjugated synthetic peptide derived from human VPS45A
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-12777R-CY5-5)?
Cy5.5 on BS-12777R-CY5-5: excitation / emission 675nm/694nm.