VPS4B Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12778R-BF594 |
| Product Name | VPS4B Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Fish |
| Predicted Reactivity | Rat, Cow, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human VPS4B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9525 |
| Swiss Prot | O75351 |
| Subcellular location | Cell membrane |
| Synonyms | 8030489C12Rik; Cell migration inducing 1; Cell migration-inducing gene 1 protein; MGC116271; MIG1; Protein SKD1; Skd1; SKD1B; Suppressor of K(+) transport growth defect 1; Suppressor of K+ transport defect 1; Vacuolar protein sorting 4 homolog B (S. cerevisiae); Vacuolar protein sorting 4 homolog B; Vacuolar protein sorting 4b; Vacuolar protein sorting-associated protein 4B; Vacuolar protein sorting-associating protein 4B; VPS4 2; VPS42; Vps4b; VPS4B_HUMAN. |
| Background | Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is VPS4B Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12778R-BF594) validated for, and at what dilution?
bs-12778R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does VPS4B Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12778R-BF594) react with?
Tested reactivity: Human, Mouse, Fish. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Pig.
How should bs-12778R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise VPS4B Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-12778R-BF594)?
KLH conjugated synthetic peptide derived from human VPS4B
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-12778R-BF594)?
AbBy Fluor-594 on bs-12778R-BF594: excitation / emission 590nm/617nm.