WAC Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12787R-Cy3-100uL |
| Product Name | WAC Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(ICC)
IF
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human WAC |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51322 |
| Swiss Prot | Q9BTA9 |
| Synonyms | 1110067P07Rik; BA48B24; BA48B24.1; BM 016; FLJ31290; KIAA1844; MGC10753; OTTHUMP00000019380; OTTHUMP00000019382; PRO1741; wac; WAC_HUMAN; WW domain containing adapter protein with coiled coil; WW domain containing adaptor with coiled coil; WW domain-containing adapter protein with coiled-coil; Wwp4. |
| Background | Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is WAC Polyclonal Antibody, Cy3 Conjugated (bs-12787R-Cy3) validated for, and at what dilution?
bs-12787R-Cy3 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does WAC Polyclonal Antibody, Cy3 Conjugated (bs-12787R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-12787R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise WAC Polyclonal Antibody, Cy3 Conjugated (bs-12787R-Cy3)?
KLH conjugated synthetic peptide derived from human WAC
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-12787R-Cy3)?
Cy3 on bs-12787R-Cy3: excitation / emission 512,550nm/570,615nm.