ZNF197 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12797R-Cy5 |
| Product Name | ZNF197 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Rat, Cow, Horse, Cat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ZNF197 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10168 |
| Swiss Prot | O14709 |
| Subcellular location | Nucleus |
| Synonyms | D3S1363E; OTTHUMP00000164119; P18; VHL associated KRAB A domain containing protein; VHLaK; Zinc finger protein 166; Zinc finger protein 197; Zinc finger protein 20; Zinc finger protein with KRAB and SCAN domains 9; ZKSCAN9; ZNF166; ZNF20. |
| Background | This gene product belongs to the zinc finger protein superfamily, members of which are regulatory proteins characterized by nucleic acid-binding zinc finger domains. The encoded protein contains 20 tandemly arrayed C2H2-type zinc fingers, a Kruppel-associated box (KRAB) domain, and a SCAN box. This transcript turns over rapidly and contains 3' UTR AUUUA motifs, which are often a hallmark of rapid turnover. It is overexpressed in some thyroid papillary carcinomas. This gene is located in a cluster of zinc finger genes at 3p21. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ZNF197 Polyclonal Antibody, Cy5 Conjugated (bs-12797R-Cy5) validated for, and at what dilution?
bs-12797R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ZNF197 Polyclonal Antibody, Cy5 Conjugated (bs-12797R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Horse, Cat.
How should bs-12797R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ZNF197 Polyclonal Antibody, Cy5 Conjugated (bs-12797R-Cy5)?
KLH conjugated synthetic peptide derived from human ZNF197
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-12797R-Cy5)?
Cy5 on bs-12797R-Cy5: excitation / emission 625,650nm/670nm.