ASPP2 Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti-ASPP2\/p53BP2 Polyclonal Antibody, Unconjugated (bs-1283R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti-ASPP2\/p53BP2 Polyclonal Antibody, Unconjugated (bs-1283R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Lane 1: Mouse spleen lysate Lane 2: Mouse thymus lysate Lane 3: Mouse prostate lysate Lane 4: Mouse intestine lysate probed with Rabbit Anti-ASPP2\/p53BP2 Polyclonal Antibody, Unconjugated (bs-1283R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.$

Lane 1: Mouse spleen lysate Lane 2: Mouse thymus lysate Lane 3: Mouse prostate lysate Lane 4: Mouse intestine lysate probed with Rabbit Anti-ASPP2\/p53BP2 Polyclonal Antibody, Unconjugated (bs-1283R) at 1:300 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.

$Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ASPP2 ) Polyclonal Antibody, Unconjugated (bs-1283R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (ASPP2 ) Polyclonal Antibody, Unconjugated (bs-1283R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ASPP2) polyclonal Antibody, Unconjugated (bs-1283R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ASPP2) polyclonal Antibody, Unconjugated (bs-1283R) 1:25, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Chicken

Overview
Catalog #	bs-1283R
Product Name	ASPP2 Polyclonal Antibody
Applications	WB ELISA IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ASPP2
Immunogen Range	1001-1128/1128
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7159
Swiss Prot	Q13625
Subcellular location	Nucleus
Synonyms	BBP; 53BP2; ASPP2; P53BP2; PPP1R13A; Apoptosis-stimulating of p53 protein 2; Bcl2-binding protein; Renal carcinoma antigen NY-REN-51; Tumor suppressor p53-binding protein 2; p53-binding protein 2; TP53BP2
Background	Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400