beta subunit Cholera Toxin Polyclonal Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12862R-PE-100uL |
| Product Name | beta subunit Cholera Toxin Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Others |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from CTXB/beta subunit Cholera Toxin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2613962 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | Cholera enterotoxin B chain; Cholera enterotoxin beta chain; Cholera enterotoxin gamma chain; Cholera enterotoxin subunit B; Cholera toxin B protein; Choleragenoid; CTX B; CTXB; TOX B; TOXB; VC1456; CHTB_VIBCH. |
| Background | The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is beta subunit Cholera Toxin Polyclonal Antibody, PE Conjugated (bs-12862R-PE) validated for, and at what dilution?
bs-12862R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12862R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise beta subunit Cholera Toxin Polyclonal Antibody, PE Conjugated (bs-12862R-PE)?
KLH conjugated synthetic peptide derived from CTXB/beta subunit Cholera Toxin
What are the excitation and emission wavelengths of the PE conjugate (bs-12862R-PE)?
PE on bs-12862R-PE: excitation / emission 496,564nm/578nm.