TUBB5 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12863R |
| Product Name | TUBB5 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Xenopus laevis |
| Predicted Reactivity | Sheep, Zebrafish |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TUBB5/Beta V Tubulin |
| Immunogen Range | 301-444/444 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; M40; TUBB1; TUBB5; tubulin beta 1 chain; Tubulin beta 4 chain; tubulin beta 5 chain; Tubulin beta; Tubulin beta chain; tubulin beta polypeptide; TBB5_HUMAN. |
| Background | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a nonexchangeable site on the alpha chain. The highly acidic C terminal region may bind cations such as calcium. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TUBB5 Polyclonal Antibody (bs-12863R) validated for, and at what dilution?
bs-12863R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TUBB5 Polyclonal Antibody (bs-12863R) react with?
Tested reactivity: Human, Mouse, Rat, Xenopus laevis. Predicted reactivity (expected from sequence homology but not yet tested): Sheep, Zebrafish.
How should bs-12863R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TUBB5 Polyclonal Antibody (bs-12863R)?
KLH conjugated synthetic peptide derived from human TUBB5/Beta V Tubulin Immunogen range: 301-444/444.