Biliverdin Reductase Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12868R-RBITC-100uL |
| Product Name | Biliverdin Reductase Polyclonal Antibody, RBITC Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Pig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BLVRA/Biliverdin Reductase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 644 |
| Subcellular location | Cytoplasm |
| Synonyms | Biliverdin IX alpha reductase; Biliverdin reductase A; BLVR A; BLVR; BLVRA; BVR A; BVR; BVRA; zinc-metalloprotein; BIEA_HUMAN. |
| Background | In human liver cytosolic fractions, four forms of biliverdin reductase have been identified, including two biliverdin-IX Beta reductases and two biliverdin-IX Alpha reductases, designated isozymes I and II and isozymes III and IV, respectively. Biliverdin reductase A (BLVRA), also designated biliverdin-IX Alpha-reductase, belongs to the GFO/iIDH/MocA family and the biliverdin reductase subfamily. The gene that encodes this cytoplasmic protein maps to chromosome 7p14-cen. BLVRA reduces biliverdin IX ?(the ?methene bridge of the open tetrapyrrole) to bilirubin with the concomitant oxidation of an NADH or NADPH cofactor (bilirubin + NADP+ = biliverdin + NADPH). BLVRA is expressed primarily in liver. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Biliverdin Reductase Polyclonal Antibody, RBITC Conjugated (bs-12868R-RBITC) validated for, and at what dilution?
bs-12868R-RBITC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Biliverdin Reductase Polyclonal Antibody, RBITC Conjugated (bs-12868R-RBITC) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig.
How should bs-12868R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Biliverdin Reductase Polyclonal Antibody, RBITC Conjugated (bs-12868R-RBITC)?
KLH conjugated synthetic peptide derived from human BLVRA/Biliverdin Reductase
What are the excitation and emission wavelengths of the RBITC conjugate (bs-12868R-RBITC)?
RBITC on bs-12868R-RBITC: excitation / emission 540nm/625nm.