outer surface protein A Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12879R-Cy5.5-100uL |
| Product Name | outer surface protein A Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Bacteria |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Borrelia burgdorferi Outer surface protein A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | Q04851 |
| Synonyms | ospA; Borreliella burgdorferi; Borrelia; Lyme disease; Lyme disease spirochete; Borreliella afzelii; Borreliella garinii; Borreliella valaisiana; Borreliella bavariensis; Borreliella japonica; Borrelia mayonii; Borreliella yangtze; Borreliella chilensis; Borreliella spielmanii; Borreliella bissettii; Borreliella californiensis; Borrelia lanei; Borreliella lusitaniae; Borreliella finlandensis; Borreliaceae; |
| Background | Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick-transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is outer surface protein A Polyclonal Antibody, Cy5.5 Conjugated (BS-12879R-CY5-5) validated for, and at what dilution?
BS-12879R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does outer surface protein A Polyclonal Antibody, Cy5.5 Conjugated (BS-12879R-CY5-5) react with?
Tested reactivity: Bacteria.
How should BS-12879R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise outer surface protein A Polyclonal Antibody, Cy5.5 Conjugated (BS-12879R-CY5-5)?
KLH conjugated synthetic peptide derived from Borrelia burgdorferi Outer surface protein A
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-12879R-CY5-5)?
Cy5.5 on BS-12879R-CY5-5: excitation / emission 675nm/694nm.