BPOZ Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-12881R-BF647-100uL |
| Product Name | BPOZ Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BPOZ/ABTB1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80325 |
| Subcellular location | Cytoplasm |
| Synonyms | ABTB 1; ABTB1; ABTB1_HUMAN; Ankyrin repeat and BTB POZ domain containing 1; Ankyrin repeat and BTB/POZ domain containing 1; Ankyrin repeat and BTB/POZ domain containing protein 1; Ankyrin repeat and BTB/POZ domain-containing protein 1; EF1ABP; Elongation factor 1A binding protein; Elongation factor 1A-binding protein; MGC20585; PP 2259; PP2259. |
| Background | ABTB1 is a 478 amino acid protein localized to the cytoplasm. ABTB1 contains two ANK repeats and two BTB (POZ) domains. The BTB (POZ) domain is thought to be involved in protein-protein interactions, and may indicate a role of ABTB1 in developmental processes. It has also been suggested that ABTB1 may be a mediator of the PTEN growth-suppressive signaling pathway. ABTB1 is ubiquitously expressed in all fetal tissues, with lower levels of expression found in adult heart. ABTB1 exists as four isoforms produced by alternative splicing. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is BPOZ Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12881R-BF647) validated for, and at what dilution?
bs-12881R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BPOZ Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12881R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Horse.
How should bs-12881R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BPOZ Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-12881R-BF647)?
KLH conjugated synthetic peptide derived from human BPOZ/ABTB1
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-12881R-BF647)?
AbBy Fluor-647 on bs-12881R-BF647: excitation / emission 650nm/665nm.