BPY2 Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-12882R-HRP-100uL |
| Product Name | BPY2 Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BPY2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 442867 |
| Subcellular location | Nucleus |
| Synonyms | Basic charge; Basic charge Y linked 2; Basic charge Y linked 2C; Basic protein on Y chromosome 2; BPY2A; BPY2B; BPY2C; Testis specic basic protein on Y 2; Testis specic basic protein Y 2; Testis-specic basic protein Y 2; Variable charge Y linked 2; Variably charged protein Y 2; VCY2; VCY2_HUMAN; VCY2A; VCY2B; VCY2C; Y-linked 2. |
| Background | Mammalian spermatogenesis is a complex developmental process. Mutations at multiple loci and in structurally and functionally disparate genes in the genome affect gametogenesis. The analysis of mutations has provided insight into biochemical pathways required for completion of this process. The basic protein on Y chromosome 2 gene (BPY2, also designated VCY2) is located in a frequently deleted azoospermia factor c region. Three copies (paralogs) of the BPY2 gene (BPY2A, BPY2B, BPY2C) reside in the AZFc region. BPY2 protein expression is localized to the nuclei of spermatogonia, spermatocytes and round spermatids, but is absent from elongated spermatids. Impaired expression of BPY2 in infertile men suggests its involvement in male germ cell development. BPY2 interacts with MAP-1S, which shares homology with microtubule-associated proteins (MAPs), suggesting a role for BPY2 within the cytoskeletal network. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is BPY2 Polyclonal Antibody, HRP Conjugated (bs-12882R-HRP) validated for, and at what dilution?
bs-12882R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BPY2 Polyclonal Antibody, HRP Conjugated (bs-12882R-HRP) react with?
Tested reactivity: Human.
How should bs-12882R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BPY2 Polyclonal Antibody, HRP Conjugated (bs-12882R-HRP)?
KLH conjugated synthetic peptide derived from human BPY2