BTB domain containing 5 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-12906R-APC-Cy7-100uL |
| Product Name | BTB domain containing 5 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Pig |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human KLHL28 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54813 |
| Subcellular location | Nucleus |
| Synonyms | BTB POZ domain containing 5; BTB/POZ domain-containing protein 5; BTBD5; FLJ20081; Kelch-like protein 28; kelchlike 28 Drosophila; KLH28_HUMAN; Klhl28; OTTHUMP00000178912; Zinc finger protein zfp47. |
| Background | KLHL28 is a 571 amino acid protein similar to the Drosophila kelch protein. KLHL26 contains six kelch repeats and one BTB (POZ) domain. The BTB (broad complex, tramtrack and bric-a-brac) domain, also known as the POZ (Poxvirus and zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. KLHL28 is expressed as two isoforms produced by alternative splicing. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is BTB domain containing 5 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12906R-APC-Cy7) validated for, and at what dilution?
bs-12906R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-12906R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BTB domain containing 5 Polyclonal Antibody, APC-Cy7 Conjugated (bs-12906R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human KLHL28
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-12906R-APC-Cy7)?
APC-Cy7 on bs-12906R-APC-Cy7: excitation / emission 650nm/780nm.