Cyr61 Polyclonal Antibody

U251 lysates probed with Cry61 Polyclonal Antibody, Unconjugated (bs-1290R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cyr61) Polyclonal Antibody, Unconjugated (bs-1290R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cyr61) Polyclonal Antibody, Unconjugated (bs-1290R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Lane 1: Human Huvec cell lysates; Lane 2: Human A431 cell lysates probed with CCN1\/Cyr61 Polyclonal Antibody, Unconjugated (bs-1290R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human Huvec cell lysates; Lane 2: Human A431 cell lysates probed with CCN1\/Cyr61 Polyclonal Antibody, Unconjugated (bs-1290R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Sheep
Horse

Overview
Catalog #	bs-1290R
Product Name	Cyr61 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Sheep, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Cyr61
Immunogen Range	251-379/379
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3491
Swiss Prot	O00622
Subcellular location	Secreted
Synonyms	CCN1; GIG1; IGFBP10; Protein CYR61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1; CYR61
Background	Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000

Applications

Reactivity

Predicted Reactivity

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